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what do you have to do so that you can see cell whit a compound microscope

Sagot :

nobodyisperfects

To see a cell with a compound microscope, follow these steps:

1. Prepare the Slide: Place a thin sample of the cell you want to observe on a glass slide. Add a drop of water or stain if needed, then cover it with a coverslip.

2. Set Up the Microscope: Turn on the microscope's light source. Place the slide on the stage and secure it with the stage clips.

3. Adjust the Objective Lens: Start with the lowest power objective lens (usually 4x or 10x). This helps you find the cell more easily.

4. Focus: Look through the eyepiece and use the coarse focus knob to move the stage up or down until the sample comes into focus. Then, use the fine focus knob for a clearer view.

5. Increase Magnification: Once you have the cell in focus, switch to a higher power objective lens (40x or 100x) for a closer look. Refocus using the fine focus knob.

And that's it! You should be able to see the cell clearly.

aqueliaz

To see cells under a compound microscope, you need to properly prepare and stain the specimen. Here are the key steps:

1. Obtain a sample of cells and place them on a clean microscope slide. This could be a wet mount of pond water, a smear of bacteria, or a thin section of tissue.

2. Fix the cells to the slide using heat or chemical fixatives like formaldehyde or ethanol. This preserves the cell structure.

3. Stain the cells with a dye that will bind to specific cellular components and make them visible. Common stains include:

  • Methylene blue - stains nucleic acids blue, making cell nuclei visible
  • Eosin - a red counterstain that binds to proteins
  • Gram stain - differentiates bacteria into gram-positive (purple) and gram-negative (red) based on cell wall composition
  • Acid-fast stain - identifies mycobacteria that resist decolorization

4. Add a coverslip to the slide, being careful to avoid air bubbles. The stain will be drawn under the coverslip.

5. Observe the stained specimen under the microscope, starting with the lowest power objective. Adjust the light, condenser, and focus to optimize contrast and resolution.

The staining process enhances the visibility of cellular structures by selectively binding dyes to certain components. This allows the shape, size, and internal organization of cells to be clearly seen under the microscope. Proper preparation and staining are essential for visualizing the fine details of cellular morphology.

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