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What is accomplished by turning the coarse adjustment (of a microscope) upwards? downwards?

Sagot :

Answer:

By turning the coarse adjustment knob of a microscope upwards or downwards, you are actually bringing the specimen into “focus”. The coarse adjustment knob which is located on the microscope’s arm elevates or lowers the stage to make the specimen into focus or clearer in view of the observer. The gearing mechanism of the adjustment creates large vertical movement of the stage with only a small revolution of the knob. Because of this, it is advised to use the coarse adjustment only with low power (4X and 10X objectives) and not with the high power lenses (40X and 100X) to prevent damage off the lenses.

Microscope

Microscope is one of the most important scientific equipment which enables us to see things that are too small to see with our naked eyes. It is and instrument that creates enlarged images of small objects, allowing the observer an exceedingly close view of minute structures at a scale convenient for examination and analysis. Medical scientists use them to see the germs that make people sick. Biologists use them to see how plants and animals are constructed. Geologists(scientists who study the Earth) use them to find out what rocks are made of. Some microscopes are so powerful that they enable us to see individual atoms, the tiny building blocks of everything.

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Types of Microscopes

  • Light Microscope
  • Stereoscope
  • Scanning Electron Microscope
  • Transmission Electron Microscope

Light Microscope - the models commonly used in most schools, use compound lenses to magnify objects. The lenses bend or refract light to make the object beneath them appear closer. Common magnifications are: 40x, 100x, 400x

Stereoscope - this microscope enables binocular (two eyes) viewing of bigger specimens.

Scanning Electron Microscope - allow scientists to view a universe too tiny to be seen with the use light microscope. Instead of using light waves; they use electrons (negatively charged electrical particles) to magnify objects up to two million times.

Transmission Electron Microscope - also uses electrons, but instead of scanning the surface (as with SEM's) electrons are passed through very thin specimens.

 

Parts of a Microscope

  • Eyepiece Lens
  • Tube
  • Arm
  • Base
  • Illuminator
  • Stage
  • Removing Nosepiece or Turret
  • Objective Lenses
  • Rack Stop
  • Condenser Lens
  • Diaphragm or Iris
  • Coarse Adjustment Knob
  • Fine Adjusment Knob

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How to Use a Microscope

  1. Rotate the revolving turret so that the lowest power objective lens (eg. 4x) is locked into position.
  2. Place the microscope slide on the stage and fasten it with the stage clips.
  3. Look at the objective lens and the stage from the side and turn the focus knob so the stage moves upward. Move it up as far as it will go without letting the objective touch the coverslip.
  4. Look through the eyepiece and move the focus knob until the image comes into focus.
  5. Adjust the condenser and light intensity for the greatest amount of light.
  6. Move the microscope slide around until the sample is in the center of the field of view (what you see).
  7. Use the focus knob to place the sample into focus and readjust the condenserand light intensity for the clearest image (with low power objectives you might need to reduce the light intensity or shut the condenser).
  8. When you have a clear image of your sample with the lowest power objective, you can change to the next objective lenses. You might need to readjust the sample into focus and/or readjust the condenser and light intensity. If you cannot focus on your specimen, repeat steps 3 through 5 with the higher power objective lens in place. Do not let the objective lens touch the slide!
  9. When finished, lower the stage, click the low power lens into position and remove the slide.

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