19 puf apud outes - UDG osny Arrange the following steps in each column by writing 1, 2, 3, 4. on the space provided. How to Make a Wet Mount How to Observe Specimens The cover slip is positioned at 45° with one side touching the edge of the water on the glass slide. _The body tube must be carefully lowered until the end of the LPO will Arter positioning the cover slip at 450, tre other edge of the almost touch the cover slip without titing the microscope. cover slip is slowly lowered until it rests on the water anci on the To shift LPO to HPO, the body tube is raised first. While looking from specimens. either side of the microscope, the revoiving nosepiece is turned to put the A medicine dropper is used to add a drop of water to the HPO in place. With the use of the fine adjustment knob the objective lens is specimen as a mounting medium to make the image of the slowly lowered until it almost touches the cover slip. While looking to the eyepiece, the coarse adjustment knob is slowly specimen clear. Prepare a sample for specimen. turned upward to raise the cojective until the specimen appears. This step is Forceps or tweezers can be used o place the specimen in the continuously done until t le specimen is seen clearly. center of a glass slide facing upwaris. The glass slide must be held firmly by the stage clips. It is important to The eraser-end of the pencil can be used to gently tap the always start with tie scanner objective before moving the LPO. cover slip to remove or minimize frapped bubbles. right hand)