Identify the step in recombinant DNA technology that is being describe in each item. Choose the letter of the correct answer from the box provided.
A Amplification using PCR
B. Insertion of Recombinant DNA into Host
C Isolation of Genetic Materiall
D. Isolation of Recombinant Celi
E. Ligation of DNA Molecules
F. Obtaining or culturing the Foreign Gene product
G. Restriction Enzymes Digestion
1. DNA was separated from other macromolecules and purified which involved enzymes such as
restriction enzymes.
2. The process of joining the cut fragment of DNA and the aut vector together using the enzyme
DNA ligase.
3. This allows separating and cutting out the digested DNA fragments.
-4. It helps to amplify a single copy or a few copies of DNA into thousands to millions of copies.
5. The recombinant DNA is introduced into a recipient host cell, mostly a bacterial cell.
6. The ultimate aim of this step is to produce a desirable protein expression.
7. It involves filtering the transformed host cell and isolating the recombinant cells from non-
recombinant cells.
8. The technique "Agarose Gel Electrophoresis' reveals the progress of this step.
